Journal: bioRxiv

Article Title: A cattle-derived human H5N1 isolate suppresses innate immunity despite efficient replication in human respiratory organoids

doi: 10.1101/2025.11.02.684669

Figure Lengend Snippet: Antiviral drug sensitivity of viruses in respiratory organoids. Respiratory organoids were infected with 200 PFU of VN1203-H5N1 or TX37-H5N1 or 100,000 PFU of Cal04-H1N1. Infected cells were incubated in the presence of DMSO, 10 µM oseltamivir acid, 20 µg/mL favipiravir, or 40 nM baloxavir acid. Culture supernatants were collected at 72 h.p.i., and virus titers were determined by plaque assay on MDCK cells. Data are presented as the mean ± s.d. (n = 3). Statistical analysis was performed using a one-way ANOVA followed by Tukey’s multiple comparisons test. The dotted line indicates the detection limit (1.0 log10 PFU/ml).

Article Snippet: The neuraminidase inhibitor oseltamivir acid and the polymerase inhibitor favipiravir (T-705) were purchased from MedChemExpress (Cat. # HY-13318) and Selleck Chemicals (Cat. # S7975), respectively.

Techniques: Infection, Incubation, Virus, Plaque Assay